Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity

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• 作者：Pinaki M ; al ; Carlos Alberto Pujol ; Elsa Beatriz Damonte ; Tuhin Ghosh ; Bimalendu Ray
• 关键词：Scinaia hatei ; Liagoraceae ; Herpes simplex virus ; Antiviral activity ; Xylan ; Sulfation
• 刊名：International Journal of Biological Macromolecules
• 出版年：2010
• 出版时间：1 March 2010
• 年：2010
• 卷：46
• 期：2
• 页码：173-178
• 全文大小：553 K

文摘

Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120 kDa and a linear structure of (1 → 4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50 % (IC₅₀) from 0.22 to 1.37 μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry.