Spermatogonial Nature of the Germ Cell Component of Canine Testicular Mixed Germ Cell-Sex Cord Stromal Tumours

详细信息    查看全文

• 作者：S. Mizukami^&lowast ; ; ^&dagger ; ; T. Murakami^&Dagger ; ; T. Tanaka^&lowast ; ; ^&dagger ; ; N. Machida^§ ; ; K. Nomura^¶ ; ; T. Yoshida^&lowast ; ; M. Shibutani^&lowast ; ; ^{mshibuta@cc.tuat.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：dog ; mixed germ cell&ndash ; sex cord stromal tumour ; protein gene product 9.5 ; Sal-like protein 4
• 刊名：Journal of Comparative Pathology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：155
• 期：1
• 页码：5-14
• 全文大小：1888 K

文摘

The present study has characterized the germ cell component of canine testicular mixed germ cell–sex cord stromal tumours (MGSCTs) by examining the histological nature and histochemical and immunohistochemical features using gonocytic and spermatogonial cellular markers, c-Kit, placental alkaline phosphatase (PLAP), protein gene product 9.5 (PGP9.5), Sal-like protein 4 (SALL4), and the periodic acid–Schiff (PAS) reaction. Histologically, all 45 examples of MGSCTs were classified as spermatocytic seminomas (SSs) and Sertoli cell tumours in combination. The germ cell component of all MGSCTs was negative by PAS staining. Immunohistochemically, PLAP immunoreactivity was lacking in the germ cell component of all MGSCTs, which is not consistent with a gonocytic origin. The germ cell component was positive for PGP9.5 and SALL4 in all MGSCTs and positive for c-Kit in 53% of MGSCTs, which is consistent with the phenotype of spermatogonia. Furthermore, the germ cell component in 71% of MGSCTs had moderate immunoreactivity for SALL4, which is suggestive of a spermatogonial phenotype. Conversely, 29% of cases had a minor population of germ cells showing strong SALL4 immunoreactivity, suggesting a phenotype similar to prespermatogonia. The results suggest that the germ cell component of canine MGSCTs is morphologically classified as SS, with the majority of cases showing the spermatogonial phenotype and some cases containing a small population of prespermatogonia.