Effect of various concentrations of butylated hydroxyanisole and butylated hydroxytoluene on freezing capacity of Turkman stallion sperm
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- 作者：Afshin Seifi-Jamadi<sup>asup> ; Hamid Kohram<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>Hamid_Kohram@yahoo.com" class="auth_mail" title="E-mail the corresponding authorsup> ; Ahmad Zareh-Shahne<sup>asup> ; Parvaneh Dehghanizadeh<sup>asup> ; Ejaz Ahmad<sup>csup>
- 关键词：Horse semen ; BHA ; BHT ; Lipid peroxidation ; Freezing ; Sperm motility
- 刊名：Animal Reproduction Science
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：170
- 期：Complete
- 页码：108-113
- 全文大小：516 K
文摘
The present study aimed to determine the effect of different concentrations of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) on post-thaw stallion sperm quality. The ejaculates collected from four healthy mature Turkmen stallions were pooled and divided into eight aliquots. The samples were diluted with extenders containing different concentrations (0.5, 1 or 2 mM/mL) of BHA or BHT. The positive control (PC) samples were diluted with extender containing 0.5% ethanol (v/v) whereas; the negative control (NC) samples were diluted with basic extender only. Semen samples were frozen according to a standard protocol. After thawing of samples, sperm motility, viability, membrane integrity, total abnormality and lipid peroxidation were assessed. The greatest (P < 0.05) values for total sperm motility, viability and plasma membrane functionality and least values for malonedialdehyde (MDA) concentration were observed in samples supplemented either with 1 mM BHT or 2 mM BHA. However, the progressive motility was greater (P < 0.05) only in samples treated with 2 mM BHA. In conclusion, the use of 1 mM BHT or 2 mM BHA in extender improves the freezing capacity of stallion sperm by reducing oxidative stress during freeze-thaw process.