The efficiency of dexamethasone sodium phosphate–encapsulated chitosan microspheres after cold injury

详细信息    查看全文

• 作者：Omer Faruk Turkoglu ; Hakan Eroglu ; Ozerk Okutan ; Ebru Burul ; Mustafa F. Sargon ; Nazmi Ö ; zer ; Levent Ö ; ner ; Etem Beskonaklı
• 关键词：Cold injury ; Brain edema ; Chitosan ; Microsphere ; Dexamethasone sodium phosphate
• 刊名：Surgical Neurology
• 出版年：2005
• 出版时间：November 2005
• 年：2005
• 卷：64
• 期：Supplement 2
• 页码：S11-S16
• 全文大小：993 K

文摘

Objective

The aims of this study were to evaluate the efficiency of dexamethasone sodium phosphate (DSP) in the treatment of cold injury–induced brain edema and to compare systemic and topical application of DSP.

Methods

Sprague-Dawley rats weighing nearly 300 g were used in the experiments. Brain edema was formed by cold injury using metal sterile rods with a diameter of 4 mm that were previously cooled at −80 °C. Twenty-four hours after the injury, animals were decapitated and brain tissues were investigated by wet-dry weight method, lipid peroxidation ratio, and histological examination.

Results

The degree of edema was significantly lowered in groups in which DSP was administered using chitosan microspheres and by intraperitoneal route (P < .05). The statistical evaluation of the experimental results was performed using Mann-Whitney U test. Histological findings transmission electron microscopy (TEM) correlated with the quantitative results.

Conclusion

Both intraperitoneal- and microsphere-administered DSP were found to be very effective in a cold injury brain edema model. The authors believe that future studies should lead to new applications of the microsphere formulations prepared by chitosan as the matrix material in many other therapies.