A real-time, fluorescence-based assay for Rho-associated protein kinase activity
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- 作者：Maia I. Kelly3 ; Tyler J. Bechtel3 ; 1 ; D. Rajasekhar Reddy2 ; Erome D. Hankore ; Jon R. Beck ; Cliff I. Stains ; cstains2@unl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Kinase activity assay ; Fluorescence-based biosensor ; Rho-associated protein kinase ; Inhibition ; Small molecule screening
- 刊名：Analytica Chimica Acta
- 出版年：2015
- 出版时间：3 September 2015
- 年：2015
- 卷：891
- 期：Complete
- 页码：284-290
- 全文大小：1190 K
文摘
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Present a novel direct activity probe for ROCK.
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Demonstrate a limit of detection of 10 pM ROCK2.
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Demonstrate the ability to screen small molecules for ROCK2 inhibitors.
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Identify two previously unreported ROCK2 inhibitors.
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Provide conditions to selectively monitor ROCK activity.