- 作者：Daniella Markovitch ; Rex M. Tyrrell ; Pedro Tauler ; Jan Frystyk ; Keith Stokes ; Dylan Thompson
- 关键词：Carotenoids ; Mononuclear cells ; Apoptosis ; Necrosis ; Insulin-like growth factors
- 刊名：Nutrition
- 出版年：2009
- 出版时间：June 2009
- 年：2009
- 卷：25
- 期：6
- 页码：668-675
- 全文大小：279 K
ObjectiveWe tested the hypothesis that lycopene supplementation reduces the expression of oxidant-responsive heme oxygenase-1 (HO-1) in basal conditions and in response to an oxidant challenge and determined whether this is temporally associated with increased cell viability.Methods
We determined basal and stimulated ex vivo expression of HO-1 and cell viability in lymphocytes from volunteers after lycopene supplementation. Twenty-four healthy young men on a low lycopene diet consumed 1) 170 g of passata sauce with butter or 2) butter alone for 3 wk in a randomized crossover design.
Results
Plasma lycopene concentrations at the end of the tomato and control trials were 0.54 ± 0.20 versus 0.20 ± 0.15 μmol/L, respectively (P < 0.05). There was a significant increase in the proportion of live cells (91 ± 5 % versus 87 ± 9 % ) and a corresponding reduction in apoptosis (6 ± 4 % versus 11 ± 9 % ) in untreated lymphocytes after supplementation (P < 0.05), with no effect on cell viability in response to hydrogen peroxide treatment. HO-1 protein expression in basal conditions and induction of HO-1 after hydrogen peroxide treatment was not different between trials.
Conclusion
Lycopene supplementation did not affect basal oxidative stress or susceptibility to oxidant-induced stress as indicated by the expression of the oxidant-responsive protein HO-1 and cell viability in response to hydrogen peroxide treatment. However, lycopene supplementation significantly reduced apoptosis in freshly harvested untreated lymphocytes. We conclude that this was not through an oxidant-mediated mechanism because of the lack of an effect on oxidant-responsive HO-1.