Effects of andrographolide and 14-deoxy-11,12-didehydroandrographolide on cultured primary astrocytes and PC12 cells

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• 作者：Yew-Min Tzeng ; Yi-Ching Lee ; Wen-Tai Cheng ; Hui-Nung Shih ; Huang-chi Wang ; Yerra Koteswara Rao ; Meng-Jen Lee ; ^{mjlee@cyut.edu.tw}
• 关键词：Andrographolide ; 14-Deoxy-11 ; 12-didehydroandrographolide ; CSPG ; Reactive oxygen species ; Astrocytes
• 刊名：Life Sciences
• 出版年：2012
• 出版时间：13 February 2012
• 年：2012
• 卷：90
• 期：7-8
• 页码：257-266
• 全文大小：1487 K

文摘

Aims

To test the effects of andrographolide (AP1) and 14-deoxy-11,12-didehydroandrographolide (AP2) on pheochromocytoma cell line 12 (PC12) cells in an astrocyte-rich environment.

Main methods

The abilities of AP1 and AP2 to reduce the secretion of pro-inflammatory cytokines Interleukin (IL)-1, IL-6, and Tumor necrosis factor (TNF)-¦Á from stimulated astrocytes were tested. In addition, the abilities of AP1 and AP2 to reduce oxidative stress in astrocytes were tested using an oxidative-sensitive fluorescent dye. The reduction of chondroitin sulfate proteoglycan (CSPG) in stimulated astrocytes was tested using the dot blot method. Reduction of H₂O₂-induced death was tested in PC12 cells. Astrocyte-conditioned medium (ACM) and TNF-¦Á-stimulated astrocyte-conditioned medium (SACM) were used to assess the effects of AP2 on PC12 cells treated with H₂O₂.

Key findings

AP1 and AP2 reduced pro-inflammatory cytokines, reactive oxygen species (ROS), and CSPG in TNF-¦Á stimulated astrocytes. AP1 protected H₂O₂-treated PC12 cells cultured in ACM. Co-incubation of PC12 cells in H₂O₂, and ACM collected from AP1 treated astrocytes did not prevent cell death.

Significance

AP1 and AP2 effectively ameliorated astrocytic pro-inflammatory reactions and prevented PC12 cell death with different efficacies. These compounds may be candidates for treatment of spinal-cord injury and neurodegeneration.