An advanced collagen aggregate composed of typical D-periodic cross-striated collagen fibrils and fiber bundles was prepared.
Ag-col could serve as a better alternative source of collagen-based material.
The thermostability and structural stability of Ag-col are much higher than that of Col.
The hierarchical architecture of Ag-col is different from that of the collagen fibrils or microfibrils reconstituted in vitro.
The secondary structural components of Ag-col and Col are similar but have some differences.