We have identified ten amoebal Pif1 helicases possessing novel accessory domains.
Domains are involved in ubiquitination, DNA replication or nucleic acid binding.
These proteins evolved via horizontal gene transfer, gene duplication and fusion.
Acanthamoeba accessory domains were acquired from fungi in two different phyla.
These domains have likely improved or expanded the roles of amoebal Pif1 helicases.