Ubiquitin-specific protease 14 regulates cell proliferation and apoptosis in oral squamous cell carcinoma

详细信息    查看全文

• 作者：Xiangyun Chen^a ; ¹ ; Jingjing Wu^a ; ¹ ; Yitian Chen^a ; ¹ ; Dongxia Ye^b ; Hu Lei^c ; ^d ; Hanzhang Xu^c ; ^d ; Li Yang^c ; ^d ; Yingli Wu ; M.D. Ph.D.^c ; ^d ; ^{wuyingli@shsmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Wenli Gu ; M.D.^a ; ^{guwl72@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ubiquitin-specific protease 14 ; Oral squamous cell carcinoma ; Expression ; Proliferation ; Apoptosis ; Migration
• 刊名：International Journal of Biochemistry and Cell Biology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：79
• 期：Complete
• 页码：350-359
• 全文大小：3009 K

文摘

Ubiquitin-specific protease 14, a deubiquitinating enzyme, has been implicated in the tumorigenesis and progression of several cancers, but its role in oral squamous cell carcinoma remains to be elucidated. The aim of this study was to explore the expression pattern and roles of Ubiquitin-specific protease 14 in the occurrence and development of oral squamous cell carcinoma. Interestingly, Ubiquitin-specific protease 14 was overexpressed in oral cancer tissues and cell lines at both mRNA and protein levels. b-AP15, a specific inhibitor of Ubiquitin-specific protease 14, significantly inhibited the growth of cancer cells and increased cell apoptosis in a dose-dependent manner. Moreover, knockdown of Ubiquitin-specific protease 14 by shRNA significantly inhibited the proliferation and migration of cancer cells in vitro. Finally, using a xenograft mouse model of oral squamous cell carcinoma, knockdown of Ubiquitin-specific protease 14 markedly inhibited tumor growth and triggered the cancer cell apoptosis in vivo, supporting previous results. In conclusion, for the first time we have demonstrated the expression pattern of Ubiquitin-specific protease 14 in oral squamous cell carcinoma and verified a relationship with tumor growth and metastasis. These results may highlight new therapeutic strategies for tumor treatment, application of Ubiquitin-specific protease 14 selective inhibitor, such as b-AP15, or knockdown by shRNA. Collectively, Ubiquitin-specific protease 14 could be a potential therapeutic target for oral squamous cell carcinoma patients.