Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus

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• 作者：Yasumitsu Uraki ; Junji Nemoto ; Hiroyuki Otsuka ; Yutaka Tamai ; Junji Sugiyama ; Takao Kishimoto ; Makoto Ubukata ; Hiroshi Yabu ; Masaru Tanaka ; Masatsugu Shimomura
• 关键词：Cellulose Iα ; Electron diffraction ; Honeycomb-patterned bacterial cellulose ; Self-organization
• 刊名：Carbohydrate Polymers
• 出版年：2007
• 出版时间：1 May 2007
• 年：2007
• 卷：69
• 期：1
• 页码：1-6
• 全文大小：825 K

文摘

Bacterial cellulose (BC)-producing bacterium, Gluconacetobacter xylinus (ATCC53582), was found to move along linear microgrooves of a stripe-patterned cellulosic scaffold. On the basis of this finding, fabrication of honeycomb-patterned BC was attempted by controlling the bacterial movement using a agarose film scaffold with honeycomb-patterned grooves (concave type). The patterned agarose film was prepared by three steps. The first was transcription of a honeycomb-patterned polycaprolactone film template with polydimethyl siloxane. When the bacteria were cultured on the scaffold under atmospheric conditions, only bacterial proliferation was observed. Honeycomb-patterned BC was obtained when cultured under a humid CO₂ atmosphere. Electron diffraction and polarized microscopic observation showed that the patterned BC comprised of the well defined cellulose Iα microfibrils.

As another attempt to fabricate honeycomb-patterned BC, the bacteria were cultured on the patterned cellulose and agarose film with convex type of honeycomb. This culture yielded no honeycomb-patterned BC. Therefore, concave type honeycomb scaffold is more suitable to fabricate honeycomb-patterned BC.