Relationship between melanin-concentrating hormone- and neuropeptide Y-containing neurons in the goldfish hypothalamus

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• 作者：Kouhei Matsuda ; Kenji Kojima ; Sei-Ichi Shimakura ; Tohru Miura ; Minoru Uchiyama ; Seiji Shioda ; Hironori Ando ; Akiyoshi Takahashi
• 关键词：Brain ; Food intake ; Goldfish ; Hypothalamus ; MCH ; Neuronal interaction ; NPY
• 刊名：Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
• 出版年：2009
• 出版时间：May 2009
• 年：2009
• 卷：153
• 期：1
• 页码：3-7
• 全文大小：537 K

文摘

Intracerebroventricular (ICV) administration of melanin-concentrating hormone (MCH) inhibits food intake in goldfish, unlike the orexigenic action in rodents, via the melanocortin system with suppression of neuropeptide Y (NPY) mRNA expression. We therefore investigated the neuronal relationship between MCH- and NPY-containing neurons in the goldfish brain, using a double-immunofluorescence method and confocal laser scanning microscopy. MCH- and NPY-like immunoreactivities were distributed throughout the brain. In particular, MCH-containing nerve fibers or endings lay in close apposition to NPY-containing neurons in a specific region of the hypothalamus, the nucleus posterioris periventricularis (NPPv). These observations suggest that MCH-containing neurons provide direct input to NPY-containing neurons in the NPPv of goldfish, and that MCH plays a crucial role in the regulation of feeding behavior as an anorexigenic neuropeptide, inhibiting the orexigenic activity of NPY.