Effect of Amalaki rasayana on DNA damage and repair in randomized aged human individuals

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• 作者：Udupi Vishwanatha^a ; ^{uvmlsc@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Kanive P. Guruprasad^a ; ^{gparashiva@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Puthiya M. Gopinath^a ; ^{gopinathpm@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Raviraj V. Acharya^b ; ^{rvacharya@manipal.edu" class="auth_mail" title="E-mail the corresponding author} ; Bokkasa V. Prasanna^c ; ^{drbvidyaprasanna@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Jayakrishna Nayak^c ; ^{nayakjk2004@yahoo.co.in" class="auth_mail" title="E-mail the corresponding author} ; Rajeshwari Ganesh^c ; ^{2009rajeshwari@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Jayalaxmi Rao^c ; ^{jamsyrao27@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Rashmi Shree^c ; ^{ayurresearch2008@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Suchitra Anchan^c ; ^{anchansuchitra@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Kothanahalli S. Raghu^a ; ^{raghushivanna@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Manjunath B. Joshi^a ; ^{joshmanjunath@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Puspendu Paladhi^a ; ^{puspendumid@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Panniampilly M. Varier^d ; ^{pmvarier@aryavaidyasala.com" class="auth_mail" title="E-mail the corresponding author} ; Kollath Muraleedharan^d ; ^{kmurali@aryavaidyasala.com" class="auth_mail" title="E-mail the corresponding author} ; Thrikovil S. Muraleedharan^d ; ^{tsmurali@aryavaidyasala.com" class="auth_mail" title="E-mail the corresponding author} ; Kapaettu Satyamoorthy^a ; ^{ksatyamoorthy@manipal.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Pyrogallol (PubChem CID ; 1057) ; Ellagic acid (PubChem CID ; 5281855) ; Gallic acid (PubChem CID ; 370) ; Ascorbic acid (PubChem CID ; 54670067) ; Citric acid (PubChem CID ; 311) ; Quercetin (PubChem CID ; 5280805) ; Kaempferol (Pubchem CID ; 5280863)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：15 September 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：387-397
• 全文大小：2365 K

文摘

Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals.

Methods

Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed.

Results

Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups.

Conclusion

Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities.