CEA-related proteins on human urothelial cell lines of different transformation grades
详细信息 查看全文
- 作者：Krop-Watorek ; Anna ; Laskowska ; Anna ; Salwa ; Jan ; Klopocki ; Arkadiusz G. ; Grunert ; Fritz ; Ugorski ; Maciej
- 关键词：CEA family proteins ; Human urothelial cell lines ; Transformation grade ; Monoclonal antibodies
- 刊名：Cancer Letters
- 出版年：1999
- 出版时间：May 3, 1999
- 年：1999
- 卷：139
- 期：1
- 页码：15-22
- 全文大小：263 K
文摘
CEA family proteins from human urothelial cell lines of different transformation grades were characterized by flow cytometry and Western blotting using monoclonal antibodies: 26/3/13, D14HD11, 9A6 and 4/3/17. The following observations were made: (i) the urothelial cell lines, representing transformation grade III (TGr III, tumorigenic, invasive cells), were characterized by the presence of a component with molecular mass 110–135 kDa, most probably representing biliary glycoprotein (BGP); (ii) BGP was absent in non-tumorigenic and non-invasive TGr II urothelial cell lines; (iii) a protein band with apparent molecular mass 180 kDa, and migrating as a CEA standard was detected in only one of seven urothelial cell lines analyzed; (iv) a broad band of apparent molecular mass migrating at 65–90 kDa, probably representing NCA-50/90, was found in two tumorigenic and invasive cell lines, HCV 29T and Hu 1703He.