Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution
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- 作者：Trushar R. Patela ; trushar7@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Donald J. Winzorb ; David J. Scottc ; d ; David.Scott@nottingham.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Analytical ultracentrifugation ; Catalytic complex in selenocysteine synthesis ; Cytoplasmic dynein complex ; Mitogen-activated protein kinase (ERK2) ; Sedimentation equilibrium ; Sedimentation velocity ; SepSecS&ndash ; tRNASec interaction
- 刊名：Methods
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：95
- 期：Complete
- 页码：55-61
- 全文大小：521 K
文摘
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Application of analytical ultracentrifuge to study macromolecular assemblies.
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Sedimentation velocity analysis to assess quality of reconstituted cytoplasmic dynein (CyDn) complex.
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Sedimentation velocity and equilibrium analysis to justify monomeric nature of activated ERK2.
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Comparison of crystal structure of tRNASec–SepSecS complex with its solution state using sedimentation velocity analysis.