Walker-A Motif Acts to Coordinate ATP Hydrolysis with Motor Output in Viral DNA Packaging
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- 作者：Damian delToro1 ; &dagger ; ; David Ortiz2 ; &dagger ; ; Mariam Ordyan1 ; Jean Sippy3 ; Choon-Seok Oh3 ; Nicholas Keller1 ; Michael Feiss3 ; michael-feiss@uiowa.edu" class="auth_mail" title="E-mail the corresponding author ; Carlos E. Catalano2 ; 4 ; carlos.catalano@ucdenver.edu" class="auth_mail" title="E-mail the corresponding author ; Douglas E. Smith1 ; des@ucsd.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：IHF ; integration host factor ; WT ; wild-type ; CD ; circular dichroism ; SV-AUC ; sedimentation velocity analytical ultracentrifugation ; DC ; defective coupling ; SA ; streptavidin
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：3 July 2016
- 年：2016
- 卷：428
- 期：13
- 页码：2709-2729
- 全文大小：1535 K
文摘
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Detailed structure–function mechanisms of viral packaging motors remain unclear.
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Study of an extensive collection of mutants shows P-loop residues play critical roles.
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Residues are implicated in ATP binding, mechanochemical coupling, and motor assembly.
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Biochemical and biophysical measurements provide support for recent structural models.
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The P-loop plays key roles in coordinating ATP hydrolysis with DNA translocation.