- 作者：Ana Paula de Almeida ; DDSc&lowast ; ; Matheus Albino Souza ; PhD&lowast ; ; matheus292@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author ; Daniela Cristina Miyagaki ; PhD&lowast ; ; Yuri Dal Bello ; MSc&lowast ; ; Doglas Cecchin ; PhD&lowast ; ; &dagger ; ; Ana Paula Farina ; PhD&lowast ; ; &dagger ;
- 关键词：Calcium hypochlorite ; Enterococcus faecalis ; passive ultrasonic irrigation ; sodium hypochlorite
- 刊名：Journal of Endodontics
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：40
- 期：12
- 页码：1953-1957
- 全文大小：225 K
Methods
The root canals of 60 single-rooted bovine extracted teeth were enlarged up to a file 45, autoclaved, inoculated with Enterococcus faecalis, and incubated for 30 days. The samples were divided into 6 groups (n = 10) according to the protocol for decontamination: G1: no treatment; G2: distilled water; G3: 2.5% NaOCl; G4: 2.5% Ca(OCl)2; G5: 2.5% NaOCl with ultrasonic activation; and G6: 2.5% Ca(OCl)2 with ultrasonic activation (US). Microbiological testing (colony-forming unit [CFU] counting) was performed to evaluate and show, respectively, the effectiveness of the proposed treatments. Data were subjected to 1-way analysis of variance followed by the post hoc Tukey test (α = 0.05).
Results
Groups 1 and 2 showed the highest mean contamination (3.26 log10 CFU/mL and 2.69 log10 CFU/mL, respectively), which was statistically different from all other groups (P < .05). Group 6 (Ca[OCl]2 + US) showed the lowest mean contamination (1.00 log10 CFU/mL), with no statistically significant difference found in groups 3 (NaOCl), 4 (Ca[OCl]2), and 5 (NaOCl + US) (P < .05).
Conclusions
Ca(OCl)2 as well as passive ultrasonic irrigation can aid in chemomechanical preparation, contributing in a significant way to the reduction of microbial content during root canal treatment.