Expression and purification of polioviral proteins in E. coli, and production of antisera as reagents for immunological assays
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- 作者：Madala Uma ; P.P. Rao ; K. Nagalekshmi ; N.R. Hegde ; hegden@ellafoundation.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：Poliovirus ; Protein expression ; Autoinduction ; Immunoassays
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：115-122
- 全文大小：1815 K
文摘
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Poliovirus structural and non-structural proteins were expressed in E. coli using autoinduction.
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Purified proteins were used for raising antibodies.
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Antisera raised could detect antigens in virus-infected cells by different immunoassays.
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Antisera could detect both cleaved and uncleaved as well as native and denatured viral proteins.
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These reagents may be useful in vaccine manufacturing and seromonitoring in the future.