Determination of topological structure of ARL6ip1 in cells: Identification of the essential binding region of ARL6ip1 for conophylline

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• 作者：Masahiro Kuroda ; Shintaro Funasaki ; Tsuyoshi Saitoh ; Yukiko Sasazawa ; Shigeru Nishiyama ; Kazuo Umezawa ; Siro Simizu
• 关键词：ARL6ip1 ; ADP-ribosylation factor-like 6-interacting protein 1 ; CNP ; conophylline ; BCNP ; biotinyl-aminoconophylline ; ER ; endoplasmic reticulum ; Gluc ; Gaussia luciferase ; TM ; transmembrane
• 刊名：FEBS Letters
• 出版年：15 November, 2013
• 年：2013
• 卷：587
• 期：22
• 页码：3656-3660
• 全文大小：646 K

文摘

Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.