- 作者：Mark A. Underwood ; Karen M. Kalanetra ; Nicholas A. Bokulich ; Zachery T. Lewis ; Majid Mirmiran ; Daniel J. Tancredi ; David A. Mills
- 关键词：Bac-TRFLP ; Bacilli-specific terminal restriction fragment-length polymorphism ; Bif-TRFLP ; Bifidobacteria-specific terminal restriction fragment-length polymorphism ; Binf ; B infantis ; Blac ; B lactis ; cfu ; Colony-forming units ; F+Binf ; Formula plus Binf ; F+Blac ; Formula plus Blac ; H+Binf ; Human milk plus Binf ; H+Blac ; Human milk plus Blac ; HMO ; Human milk oligosaccharides ; NEC ; Necrotizing enterocolitis ; PBS ; Phosphate-buffered saline ; PCoA ; Principal coordinate analysis ; qPCR ; Quantitative polyme
- 刊名：The Journal of Pediatrics
- 出版年：December, 2013
- 年：2013
- 卷：163
- 期：6
- 页码：1585-1591.e9
- 全文大小：2925 K
Objective
To determine the impact of 2 probiotic bifidobacteria on the fecal microbiota of premature infants fed either human milk or formula.Study design
In the first of two phase 1 clinical trials, 12 premature infants receiving formula feedings were assigned randomly to receive either Bifidobacterium longum ssp infantis or Bifidobacterium animalis ssp lactis in increasing doses during a 5-week period. In the second, 9 premature infants receiving their mother's milk received each of the two bifidobacteria for 2 weeks separated by a 1-week washout period. Serial stool specimens from each infant were analyzed by terminal restriction fragment-length polymorphism and quantitative polymerase chain reaction for bacterial composition.
Results
Among the formula-fed infants, there was a greater increase in fecal bifidobacteria among infants receiving B infantis (Binf) than those receiving B lactis (Blac). This difference was most marked at a dose of 1.4聽脳 109 colony-forming units twice daily (P < .05). Bacterial diversity improved over dose/time in those infants receiving Binf. Among the human milk-fed infants, greater increases in fecal bifidobacteria and decreases in 纬-Proteobacteria followed the administration of Binf than Blac. The B longum group (which includes Binf but not Blac) was the dominant bifidobacteria among the human milk-fed infants, regardless of the probiotic administered.
Conclusions
Binf was more effective at colonizing the fecal microbiota than Blac in both formula-fed and human milk-fed premature infants. The combination of human milk plus Binf resulted in the greatest fecal levels of bifidobacteria.