Protein unfolding is essential for cleavage within the α-helix of a model protein substrate by the serine protease, thrombin
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- 作者：Amy L. Robertsona ; Stephen J. Headeyb ; Natasha M. Nga ; Lakshmi C. Wijeyewickremaa ; c ; Martin J. Scanlonb ; Robert N. Pikea ; c ; 1 ; r.pike@latrobe.edu.au" class="auth_mail" title="E-mail the corresponding author ; Stephen P. Bottomleya ; 1
- 关键词：Protease ; Cleavage ; α-helix ; Protein substrate ; Thrombin
- 刊名：Biochimie
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：122
- 期：Complete
- 页码：227-234
- 全文大小：1681 K
文摘
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Thrombin cleaved protein G with a suitable sequence within the α-helix.
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Thrombin did not induce unfolding in the helix in an enzyme–substrate complex.
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The rate of cleavage correlated with the instability of the substrate.
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This suggests that the enzyme only cleaves the unfolded substrate.