Functional disruption of IEX-1 expression by concatemeric hammerhead ribozymes alters growth properties of 293 cells
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- 作者：Grobe ; Olaf ; Arlt ; Alexander ; Ungefroren ; Hendrik ; Krupp ; Guido ; Fö ; lsch ; Ulrich R. ; Schmidt ; Wolfgang E. ; et. al.
- 关键词：Cell cycle ; Apoptosis ; Antisense ; Hammerhead ribozyme ; IEX-1 ; immediate early gene on X-radiation 1 ; MTS ; 3-(4 ; 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt ; PI ; propidium iodide ; FCS ; foetal calf serum ; DMEM ; Du
- 刊名：FEBS Letters
- 出版年：2001
- 出版时间：April 13, 2001
- 年：2001
- 卷：494
- 期：3
- 页码：196-200
- 全文大小：325 K
文摘
The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably transfected with a concatemeric ribozyme construct. In addition, these 293 cells were much less sensitive to apoptosis induced by an activating Fas/CD95 antibody or by the anti-cancer drugs etoposide and doxorubicin. By modulating the cell cycle, IEX-1 might be part of a growth signal if favourable growth conditions prevail, whereas under unfavourable conditions, i.e. death receptor activation, IEX-1 facilitates apoptosis.