Stool specimens of 1 204 children were collected; oocysts of Cryptosporidium were identified with auramine O- modified staining, acid-fast staining, safranine T and methylene blue staining, and auramine O-modified acid-fast staining.
The detectable rate of Cryptosporidium in four stainings were respectively 2.46 % , 1.50 % , 1.98 % and 3.46 % , and there was a higher significant difference in the rate between auramine O-modified acid-fast staining and the others(P <0.005).Thedetectableratewas significantly lower in urban children(2.14 % , 15/684) than in rural ones(5.19 % , 27/520). Boys and girls had similar detectable rate (1.99 % , 24/1 204 vs. 1.50 % , 18/1 204). Cryptosporidium infection was usually subclinical, and its major clinical features included benign diarrhea, mild abdominal pain and nausea.
Cryptosporidium infection was relatively common in kindergartens and a higher infection rate was found in rural children. As the majority of the Cryptosporidium infections were subclinical, diagnosis is important although difficult.