Molecular cloning of follicle-stimulating hormone (FSH)-β subunit cDNA from duck pituitary
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- 作者：San-Tai Shen ; Yi-Sheng Cheng ; Tzu-Yun Shen and John Yuh-Lin Yu
- 关键词：Follicle-stimulating hormone (FSH) ; cDNA Cloning ; Duck ; Pituitary gland ; Structural model analysis
- 刊名：General and Comparative Endocrinology
- 出版年：2006
- 出版时间：15 September 2006
- 年：2006
- 卷：148
- 期：3
- 页码：388-394
- 全文大小：894 K
文摘
We have cloned FSH-β cDNA from duck pituitary gland by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods. The cloned duck FSH-β cDNA contains 1909-bp nucleotides including 396-bp of open-reading frame and 1491-bp of 3′-untranslational region. The open-reading frame encodes a 131-amino acid protein with a putative 20-amino acid signal peptide and a putative 111-amino acid mature protein. The deduced amino acid sequence shows a remarkable similarity (94–98 % ) to those of other avian FSH-β subunits; while it exhibits lower similarities with those of turtles (82–84 % ), mammals (63–71 % ), and amphibians (53–57 % ). The structural model analysis of duck FSH suggests that the cysteine-knot and β-strands for maintaining the specific structural frame, and the “seat-belt” loop for specific binding to FSH receptor have been conserved in tetrapodian FSH-βs.