Histone deacetylases differentially regulate the proliferative phenotype of mouse bone marrow stromal and hematopoietic stem/progenitor cells
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- 作者：Neha R. Dhokea ; b ; Evangelene Kalabathulaa ; Komal Kaushika ; b ; Ramasatyaveni Geesalaa ; b ; B. Sravania ; Amitava Dasa ; b ; amitavadas@iict.res.in" class="auth_mail" title="E-mail the corresponding author ; drdas.amitava@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：BMSCs ; bone marrow stromal stem/progenitor cells ; HSPCs ; hematopoietic stem/progenitor cells ; MACS ; magnetic activated cell sorting ; LSK ; lineage ; Sca-1 ; c-Kit ; HDAC ; histone deacetylases ; CDKs ; cyclin dependent kinases ; CKIs ; cyclin dependent kinase inhibitors ; FBS ; fetal bovine serum ; MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyl tetrazolium bromide ; BrdU ; bromodeoxy uridine ; PI ; propium iodide ; G0/G1 Phase ; gap 1 phase of the cell cycle ; G2/M Phase ; gap 2 phase and mitotic phase of the cell
- 刊名：Stem Cell Research
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：17
- 期：1
- 页码：170-180
- 全文大小：2367 K
文摘
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Proliferating BMSCs expresses relatively higher cyclins B1 and D2 than HSPCs.
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HDACs are differentially expressed in BMSCs and HSPCs.
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HDAC inhibition potentiates BMSC and HSPC proliferation by increasing AcH3 and AcH4 and cyclins B1 and D2.
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TSA decrease binding of HDAC2 and 3 on cyclins B1 and D2 promoter.