Kidney stone matrix proteins ameliorate calcium oxalate monohydrate induced apoptotic injury to renal epithelial cells

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• 作者：Shifa Narula^a ; Simran Tandon^b ; Shrawan Kumar Singh^c ; Chanderdeep Tandon^a ; ^{ctandon@amity.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Nephrolithiasis ; Calcium oxalate monohydrate (COM) ; Kidney stones ; Matrix proteins ; Modulators
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：1 November 2016
• 年：2016
• 卷：164
• 期：Complete
• 页码：23-30
• 全文大小：1484 K

文摘

Kidney stone formation is a highly prevalent disease, affecting 8–10% of the human population worldwide. Proteins are the major constituents of human kidney stone's organic matrix and considered to play critical role in the pathogenesis of disease but their mechanism of modulation still needs to be explicated. Therefore, in this study we investigated the effect of human kidney stone matrix proteins on the calcium oxalate monohydrate (COM) mediated cellular injury.

Main methods

The renal epithelial cells (MDCK) were exposed to 200 μg/ml COM crystals to induce injury. The effect of proteins isolated from human kidney stone was studied on COM injured cells. The alterations in cell-crystal interactions were examined by phase contrast, polarizing, fluorescence and scanning electron microscopy. Moreover, its effect on the extent of COM induced cell injury, was quantified by flow cytometric analysis.

Key findings

Our study indicated the antilithiatic potential of human kidney stone proteins on COM injured MDCK cells. Flow cytometric analysis and fluorescence imaging ascertained that matrix proteins decreased the extent of apoptotic injury caused by COM crystals on MDCK cells. Moreover, the electron microscopic studies of MDCK cells revealed that matrix proteins caused significant dissolution of COM crystals, indicating cytoprotection against the impact of calcium oxalate injury.

Significance

The present study gives insights into the mechanism implied by urinary proteins to restrain the pathogenesis of kidney stone disease. This will provide a better understanding of the formation of kidney stones which can be useful for the proper management of the disease.