13C metabolic flux analysis of microbial and mammalian systems is enhanced with GC-MS measurements of glycogen and RNA labeling
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- 作者：Christopher P. Long1 ; Jennifer Au1 ; Jacqueline E. Gonzalez ; Maciek R. Antoniewicz ; mranton@udel.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pentose phosphate pathway ; Stable-isotope labeling ; Flux estimation ; Metabolism ; Glucose and xylose co-utilization
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：38
- 期：Complete
- 页码：65-72
- 全文大小：1322 K
文摘
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A method is presented to measure isotopic labeling of glycogen and RNA.
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Isotopic labeling of glycogen and RNA greatly enhances resolution of metabolic fluxes.
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Net and exchange fluxes in pentose phosphate pathway are precisely measured.
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Fluxes during co-utilization of glucose and xylose are precisely measured.
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Turnover rates of glycogen and RNA in CHO cells are determined.