Formation of xylitol and xylitol-5-phosphate and its impact on growth of d-xylose-utilizing Corynebacterium glutamicum strains
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- 作者：Andreas Radek ; Moritz-Fabian Mü ; ller ; Jochem Gä ; tgens ; Lothar Eggeling ; Karin Krumbach ; Jan Marienhagen ; Stephan Noack ; s.noack@fz-juelich.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Corynebacterium glutamicum ; Xylitol ; Xylitol-5-phosphate ; d-xylose ; Isomerase pathway ; Weimberg pathway
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 August 2016
- 年：2016
- 卷：231
- 期：Complete
- 页码：160-166
- 全文大小：814 K
文摘
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Corynebacterium glutamicum accumulates extracellular xylitol during aerobic growth on d-xylose containing substrates.
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Intracellular xylitol is partly converted to cytotoxic xylitol-5-phosphatein C. glutamicum pEKEx3-xylAXc-xylBCg (ISO).
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Low amounts of supplemented xylitol strongly inhibit the growth of the ISO strain on d-xylose, d-glucose and d-arabitol.
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Xylitol is a suitable substrate of the endogenous xylulokinase(encoded by xylB).
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The Weimbergroute, operating independently from xylulokinase activity, could be an effective substitute towards efficient D-xylose utilization in C. glutamicum.