Efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered Escherichia coli

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• 作者：Rongming Liu ; Liya Liang ; Feng Li ; Mingke Wu ; Kequan Chen ; Jiangfeng Ma ; Min Jiang ; Ping Wei ; Pingkai Ouyang
• 关键词：ATP ; Escherichia coli ; Succinic acid ; Simultaneous utilization ; Lignocellulosic hydrolysate
• 刊名：Bioresource Technology
• 出版年：December, 2013
• 年：2013
• 卷：149
• 期：Complete
• 页码：84-91
• 全文大小：967 K

文摘

To enhance succinic acid formation during xylose fermentation in Escherichia coli, overexpression of ATP-forming phosphoenolpyruvate carboxykinase (PEPCK) from Bacillus subtilis 168 in an ldhA, pflB, and ppc deletion strain resulted in a significant increase in cell mass and succinic acid production. However, BA204 displays a low yield of glucose fermentation and sequential glucose-xylose utilization under regulation by the phosphotransferase system (PTS). To improve the capability of glucose fermentation and simultaneously consume sugar mixture for succinic acid production, a pflB, ldhA, ppc, and ptsG deletion strain overexpressing ATP-forming PEPCK, named E. coli BA305, was constructed. As a result, after 120 h fed-batch fermentation of sugarcane bagasse hydrolysate, the dry cell weight and succinic acid concentration in BA305 were 4.58 g L^鈭? and 39.3 g L^鈭?, respectively.