Development of oral DNA vaccine based on chitosan nanoparticles for the immunization against reddish body iridovirus in turbots (Scophthalmus maximus)
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- 作者：Fengrong Zhenga ; zhengfr@fio.org.cn" class="auth_mail" title="E-mail the corresponding author ; Hongzhan Liub ; Xiuqin Suna ; Yongqiang Zhanga ; Baiyu Zhanga ; Zhaojun Tengc ; Yongjiang Houc ; Bo Wanga ; ousun@fio.org.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Turbot reddish body iridovirus ; Eukaryotic cell ; Plasmid DNA chitosan nanoparticles (pDNA-CS-NPs) ; Immune responses ; Scophthalmus maximus
- 刊名：Aquaculture
- 出版年：2016
- 出版时间：1 February 2016
- 年：2016
- 卷：452
- 期：Complete
- 页码：263-271
- 全文大小：1296 K
文摘
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Turbot reddish body iridovirus (TRBIV) is a new piscine iridovirus, hampers the development of turbot aquaculture.
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An oral nanoparticle-based DNA vaccine against TRBIV was developed to evaluate the immunization effectiveness in turbots
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pDNA-CS-NPs were spherical shape with the particle size and zeta potential of 145.5 nm and 24.3, respectively
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pDNA-CS-NPs clearly induced an innate and specific immune response, and up-regulated the expression of immune-related genes.
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Strong protection with relative survival rate of 68% was recorded in the orally-vaccinated turbots.