In vitro evaluation of poly(ethylene glycol)-block-poly(ɛ-caprolactone) methyl ether copolymer coating effects on cells adhesion and proliferation

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• 作者：Laurentiu Rusen^a ; Patricia Neacsu^b ; Anisoara Cimpean^b ; Ion Valentin^a ; Simona Brajnicov^a ; L.N. Dumitrescu^a ; Janina Banita^c ; ^d ; Valentina Dinca^a ; ^{valentina.dinca@inflpr.ro" class="auth_mail" title="E-mail the corresponding author} ; Maria Dinescu^a
• 关键词：PEG-PCL Me copolymer ; Bio-coating ; Degradation ; In vitro preosteoblast testing
• 刊名：Applied Surface Science
• 出版年：2016
• 出版时间：30 June 2016
• 年：2016
• 卷：374
• 期：Complete
• 页码：23-30
• 全文大小：3330 K

文摘

Understanding and controlling natural and synthetic biointerfaces is known to be the key to a wide variety of application within cell culture and tissue engineering field. As both material characteristics and methods are important in tailoring biointerfaces characteristics, in this work we explore the feasibility of using Matrix Assisted Pulsed Laser Evaporation technique for obtaining synthetic copolymeric biocoatings (i.e. poly(ethylene glycol)-block-poly(ɛ-caprolactone) methyl ether) for evaluating in vitro Vero and MC3T3-E1 pre-osteoblasts cell response. Characterization and evaluation of the coated substrates were carried out using different techniques. The Fourier transform infrared spectroscopy data demonstrated that the main functional groups in the MAPLE-deposited films remained intact. Atomic Force Microscopy images showed the coatings to be continuous, with the surface roughness depending on the deposition parameters. Moreover, the behaviour of the coatings in medium mimicking the pH and temperature of the human body was studied and corelated to degradation. Spectro-ellipsometry (SE) and AFM measurements revealed the degradation trend during immersion time by the changes in coating thickness and roughness. In vitro biocompatibility was studied by indirect contact tests on Vero cells in accordance with ISO 10993-5/2009. The results obtained in terms of cell morphology (phase contrast microscopy) and cytotoxicity (LDH and MTT assays) proved biocompatibility. Furthermore, direct contact assays on MC3T3-E1 pre-osteoblasts demonstrated the capacity of all analyzed specimens to support cell adhesion, normal cellular morphology and growth.