- 作者：Ana Correa-Ruiz ; Rosa Gir贸n ; Buenaventura Buend铆a ; M. Jos茅 Medina-Pascual ; Claudia Valenzuela ; Manuel L贸pez-Brea ; Juan Antonio S谩ez-Nieto
- 关键词：Burkholderia cepacia complex ; Cystic fibrosis ; Lung function ; Molecular methods
- 刊名：Enfermedades Infecciosas y Microbiolog铆a Cl铆nica
- 出版年：December, 2013
- 年：2013
- 卷：31
- 期：10
- 页码：649-654
- 全文大小：367 K
Introduction
Burkholderia cepacia complex have emerged as significant pathogens in cystic fibrosis (CF) patients due to the risk of cepacia syndrome and the innate multi-resistance of the microorganisms to antibiotics. The aim of this study was to describe the antimicrobial susceptibility profiles, the genotypes and subtypes of BCC, and the clinical evolution of CF patients with BCC.Methods
The lung function and Brasfield and Shwachman score were assessed in 12 patients. BCC were identified and susceptibility was studied by MicroScan (Siemens). Species and genospecies of BCC were confirmed by molecular methods in a Reference Centre (Majadahonda).
Results
BCC were identified in 12 of 70 patients (17.1%) over a ten year period. The mean age to colonization by BCC was 24.4 years (SD: 7.71). B. cenocepacia was isolated in 4 patients (33.3%), B. contaminans was isolated in 3 patients (25%), both B. vietnamiensis and B. stabilis were isolated in 2 patients (16.7%), and B. cepacia, B. multivorans and B. late were isolated in one patient (8.3%). Among the B. cenocepacia, subtype IIIa was identified in two strains, and subtype IIIb was identified in the other two strains. There was susceptibility to meropenem in 90% of BCC, 80% to cotrimoxazole, 60% to minocycline, 50% to ceftazidime, and 40% to levofloxacin.
Conclusions
B. cenocepacia was the most prevalent species among the BCC isolated in CF adult patients, and subtypes IIIa and IIIb were identified in the 50% of the strains. Meropenem and cotrimoxazole showed the best activity.