A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation

详细信息    查看全文

• 作者：Nadia Woitovich Valetti ; Guillermo Pic贸
• 关键词：POD ; peroxidase ; HRP ; horseradish peroxidase ; EuL ; Eudragit L 100 ; EuS ; Eudragit S100
• 刊名：Separation and Purification Technology
• 出版年：19 November, 2013
• 年：2013
• 卷：119
• 期：Complete
• 页码：1-6
• 全文大小：1111 K

文摘

The separation of radish peroxidase from a fresh Raphanus sativus L extract was carried out using precipitation with two commercially available negatively charged synthetic polyelectrolytes: Eudragit庐 L 100 and Eudragit庐 S 100. The enzyme was precipitated by polyelectrolyte addition at pH 4.00. The non-soluble complex formed was separated by simple centrifugation and re-dissolved by a pH change. The recovery of radish peroxidase biological activity was 50% of the initial activity in the homogenate for EuL and 45% for EuS, with 1.5-fold increase in its specific activity. The total Eudragit庐 concentration to precipitate the enzyme was very low: about 2 脳 10^鈭?% w/v. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up to 10 times.