Application of a LC-MS/MS method for evaluating lung penetration of tobramycin in rats by microdialysis

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• 作者：P.M. Bernardi^a ; ^b ; F. Barreto^c ; T. Dalla Costa^a ; ^b ; ^{dalla.costa@ufrgs.br}
• 关键词：Tobramycin ; Bioanalytical method validation ; LC&ndash ; MS/MS ; Tissue penetration ; Microdialysis ; Pre-clinical pharmacokinetics
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：5 February 2017
• 年：2017
• 卷：134
• 期：Complete
• 页码：340-345
• 全文大小：686 K
• 卷排序：134

文摘

A sensitive, precise and accurate LC–MS/MS method was validated to quantify to bramycin in plasma and microdialysate samples. A very low injection volume (4 μL) was used and a short retention time (∼6 min) was obtained. Microdialysis probe relative recovery for tobramycin is 30% in vitro and in vivo for a flow rate of 1.0 μL min−1. Tobramycin relative recovery is independent of drug concentration and technique used (dialysis or retrodialysis). Tobramycin has a high penetration in interstitial fluid with similar free lung and plasma concentrations in healthy rats.