- 作者：M.A. Mu?oz-S¨¢nchez ; J.J. Egea-Guerrero ; J. Revuelto-Rey ; Manuel Moreno-Valladares ; F. Murillo-Cabezas
- 关键词：SAH ; Subarachnoid Haemorrhage ; SV ; symptomatic vasospasm ; CSF ; cerebrospinal fluid
- 刊名：Journal of Neuroscience Methods
- 出版年：2012
- 出版时间：15 October, 2012
- 年：2012
- 卷：211
- 期：1
- 页码：88-93
- 全文大小：1058 K
Objective
Describe the results obtained with a new percutaneous, intracisternal model of Subarachnoid Haemorrhage (SAH) in Wistar rats by a single injection of non-heparinised, autologous blood.Methods
Once anaesthetized the rat was fixed prone in a stereotaxic frame. After identifying the projection of the occipital bone, the needle of the stereotaxic frame aspirated towards the foramen magnum until it punctured through the atlanto-occipital membrane and obtained cerebrospinal fluid. Autologous blood (100 ¦Ìl) was withdrawn from the tail and injected intracisternally. This procedure was repeated in the sham group, injecting 100 ¦Ìl of isotonic saline. On the fifth day post-intervention, the rats were anaesthetized and the brain was exposed. After a lethal injection of ketamine the brain was explanted and fixed in paraformaldehyde. Gross and microscopic inspection of the slices revealed the existence or non-existence of pathological findings.
Results
A total of 26 rats were operated on (13 in the SAH group/13 in the sham group). The average time between obtaining the blood and the start of the intracisternal injection was 10 (¡À1.2) s. The mortality rate was 16.12 % . Intra- and extraparenchymal ischemic-haemorrhagic lesions were found in three animals (23.07 % ) - all from the SAH group - with ischemic neuronal cell injury detected in two of the three.
Conclusions
The new murine model of SAH is easy to perform, with low mortality, minimally invasive, which makes it interesting for future studies on vasospasm-related delayed SAH complications.