Analytical applications of the optical properties of ferric hemoglobin: A theoretical and experimental study

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• 作者：Vanesa Sanz ; Susana de Marcos ; Javier Galb谩n
• 关键词：Ferric hemoglobin ; Autoindicating absorption properties ; Mathematical model ; Hydrogen peroxide ; Peracetic acid ; Glucose/glucose oxidase
• 刊名：Microchemical Journal
• 出版年：May, 2014
• 年：2014
• 卷：114
• 期：Complete
• 页码：175-181
• 全文大小：450 K

文摘

Like other hemeproteins, ferric hemoglobin (HbIII) not only can act as a catalyzer in the oxidation of substrates by H₂O₂ but also can act alone as a H₂O₂ reducer. In this paper, the kinetic mechanism of the reaction between HbIII and H₂O₂ is studied, and a mathematical model is developed, which permits the determination of the kinetic constants from the absorbance measurement; the results indicate that each HbIII molecule is able to oxidize up to 5 H₂O₂ molecules without external regeneration. Second, it is demonstrated that the optical properties of HbIII can also be used as an indicator of this oxidation reaction, and analytical methods can be developed using this approach. A method for H₂O₂ peroxide determination is presented, which permits the analyte to be determined in a concentration range that depends on the HbIII concentration used and the measurement time, with an RSD better than 5%. In addition, methods for peracetic acid (PPA), the simultaneous determination of PAA + H₂O₂ and the use of HbIII/H₂O₂ as an indicator for oxidase enzymatic reactions (glucose/glucose oxidase) are outlined.