A mutation in the PSST homologue of complex I (NADH:ubiquinone oxidoreductase) from Tetranychus urticae is associated with resistance to METI acaricides

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• 作者：Sabina Bajda^a ; Wannes Dermauw^b ; Rafaela Panteleri^c ; Naoya Sugimoto^d ; Vassilis Douris^c ; ^e ; Luc Tirry^b ; Masahiro Osakabe^d ; John Vontas^c ; ^f ; Thomas Van Leeuwen^a ; ^b ; ^{thomas.vanleeuwen@ugent.be}
• 关键词：20 kDa subunit ; NUKM ; METI-I ; Rotenone ; Acari ; Drosophila
• 刊名：Insect Biochemistry and Molecular Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：80
• 期：Complete
• 页码：79-90
• 全文大小：1079 K
• 卷排序：80

文摘

Target-site resistance of Tetranychus urticae to METI-I acaricides, inhibiting mitochondrial complex I, was investigated. A H92R substitution was identified in the PSST homologue of complex I from T. urticae in METI-I acaricide resistant strains. 3D-modeling revealed that H92R is located near the presumed binding site of ubiquinone and well-known complex I inhibitors. Genetic mapping and QTL analysis identified the genomic locus of pyridaben resistance, which included the PSST gene. Selection experiments and marker-assisted back-crossing showed that H92R is associated with METI-I resistance in T. urticae.