- 作者：Yogesh K. Vashist ; M.D. ; vashist@uke.de ; Guentac Uzunoglu ; M.D. ; Lena Deutsch ; M.D. ; Viacheslav Kalinin ; Ph.D. ; Oliver Zehler ; M.D. ; Adil Alzadjali ; M.D. ; Asad Kutup ; M.D. ; Jakob R. Izbicki ; M.D. ; F.A.C.S. ; Emre F. Yekebas ; M.D.
- 关键词：pancreatic neuroendocrine tumors ; polymorphism ; heme oxygenase-1 ; prognostic marker ; recurrence ; survival
- 刊名：Journal of Surgical Research
- 出版年：2011
- 出版时间：April, 2011
- 年：2011
- 卷：166
- 期：1
- 页码：e121-e127
- 全文大小：1K
Background
Current available preoperative diagnostic workup is insufficient to differentiate between benign and malignant pancreatic neuroendocrine tumors (PNET). The aim of the present study was to evaluate the potential prognostic role of the promoter GTn repeat polymorphism (GTn) of the heme oxygenase-1 gene in PNET.Methods
Tumor, metastasis, corresponding healthy tissue, and peripheral blood leukocyte DNA of 46 patients who underwent surgical resection for PNET were analyzed for GTn by PCR, capillary electrophoresis, and DNA-sequencing. The GTn was correlated to clinicopathologic parameters and clinical outcome.
Results
GTn was classified into short (<25) and long (?5) alleles and three (SS, SL, and LL) genotypes were defined. There was no difference in GTn genotype among tumor, healthy tissue, metastasis, and peripheral blood leukocyte DNA. The SS and SL genotype displayed significantly more poor differentiated tumors (P = 0.001) and higher tumor recurrence rate (P = 0.0001) compared with LL patients. The LL genotype patients presented predominantly benign PNET (P?<?0.001). The LL genotype had the longest disease-free (P < 0.001) and overall survival (P = 0.006). Besides the WHO classification the GTn was identified as a strong predictor of tumor recurrence (hazard ratio 3.1, 95 % confidence interval 1.3-7.3) in PNET.
Conclusion
GTn differentiates between benign and malignant PNET and is a strong predictor of tumor recurrence.