Faecal shedding of Mycobacterium avium subspecies paratuberculosis reduces before parturition in sheep?

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• 作者：Antonia Mataragka^a ; Elisavet Leousi^a ; Emmanouil Liandris^a ; Vasileios Ntafis^a ; Leonidas Leontides^b ; Elisavet Aggelidou^b ; Ioannis Bossis^a ; Kostas A. Triantaphyllopoulos^c ; Ioanna Theodoropoulou^a ; John Ikonomopoulos^a ; ^{ikonomop@aua.gr}
• 关键词：Paratuberculosis ; Parturition ; Shedding of MAP ; Real time PCR ; ELISA
• 刊名：Small Ruminant Research
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：147
• 期：Complete
• 页码：32-36
• 全文大小：520 K
• 卷排序：147

文摘

In sheep with no signs of clinical paratuberculosis Faecal real time PCR applied for the detection of MAP DNA before parturition may result to significant underestimation of positivity compared to the periods after parturition and before the mating season. Real time PCR performed on one faecal sample collected from each animal after parturition, detected 71.4% of all PCR-positive animals. ELISA-testing resulted to an underestimation of positivity of 57%, referenced to faecal real time PCR. The selection of the period before parturition for the detection of shedders by faecal real time PCR is probably not ideal.