Calmodulin kinase II inhibition disrupts cardiomyopathic effects of enhanced green fluorescent protein

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• 作者：Michelle S.C. Khoo ; Chad E. Grueter ; Mesut Eren ; Jinying Yang ; Rong Zhang ; Martha A. Bass ; Seint T. Lwin ; Lisa A. Mendes ; Douglas E. Vaughan ; Roger J. Colbran ; Mark E. Anderson
• 关键词：Calmodulin kinase II ; Cardiomyopathy ; eGFP
• 刊名：Journal of Molecular and Cellular Cardiology
• 出版年：2008
• 出版时间：February 2008
• 年：2008
• 卷：44
• 期：2
• 页码：405-410
• 全文大小：542 K

文摘

Transgenic expression of enhanced green fluorescent protein (eGFP) in myocardium can result in cardiac dysfunction and cardiomyopathy, presumably through toxic effects that disrupt normal cellular signaling. The multifunctional Ca²⁺- and calmodulin-dependent protein kinase II (CaMKII) is widely expressed in myocardium and CaMKII activity is increased in human and animal models of cardiomyopathy, so we hypothesized that increased CaMKII activity is important for cardiomyopathy due to transgenic expression of eGFP. Here we report that cardiomyocyte-delimited eGFP over-expression causes increased CaMKII activity that predicts left ventricular dilation and dysfunction. On the other hand, transgenic co-expression of a CaMKII inhibitory peptide with eGFP prevents eGFP-mediated left ventricular dilation and dysfunction. These findings suggest that increased CaMKII activity is a critical pathological signal in transgenic cardiomyopathy due to eGFP over-expression.