- 作者：S.C.M. de Crom ; C.C. Obihara ; R.A. de Moor ; E.J.M. Veldkamp ; A.M. van Furth ; J.W.A. Rossen
- 关键词：CSF ; cerebrospinal fluid ; EV ; human non-polio enterovirus ; HEp-2 ; human epidermoid cancer ; HPeV ; human parechovirus ; PPV ; positive predictive value ; NPV ; negative predictive value ; RNA ; ribonucleic acid ; RT-qPCR ; reverse-transcriptase real time quantitati
- 刊名：Journal of Clinical Virology
- 出版年：2013
- 出版时间：October, 2013
- 年：2013
- 卷：58
- 期：2
- 页码：449-454
- 全文大小：515 K
Background
Reverse-transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) has become the gold standard for the diagnosis of human enterovirus (EV) and parechovirus (HPeV) infections. The detection rate of RT-qPCR in different pediatric body specimens has not been compared prospectively in a multicentre study.Objectives
This study compared the diagnostic detection rates of EV and HPeV RT-qPCR and viral culture in different specimens (feces, nasopharynx, blood, urine and cerebrospinal fluid (CSF)) of pediatric patients.
Study design
This prospective, multicenter study performed an EV and HPeV RT-qPCR on nasopharynx, blood, urine, feces and CSF specimens and a viral culture on nasopharynx, feces and CSF specimens in symptomatic children < 16 years.
Results
Of 285 included children EV was detected in 140 (49 % ) and HPeV in 44 (15 % ) children. Both EV and HPeV RT-qPCR had a higher sensitivity and negative predictive value than EV and HPeV viral culture, respectively. EV and HPeV RT-qPCR in feces specimen had the highest sensitivity (99.2 % and 95.1 % ) of all specimens. Pooling results of specimens increased the detection rate for both viruses.
Conclusion
Of all specimens, RT-qPCR in feces had the highest detection rate for both EV and HPeV in symptomatic pediatric patients. An EV was detected in all EV positive patients if a RT-qPCR was performed on both feces and CSF specimens or in both feces and urine specimens. HPeV was detected in all HPeV positive patients if a RT-qPCR was performed on both feces and CSF specimens, feces and nasopharynx specimens or CSF and nasopharynx specimens.