Co-production of two new peptide antibiotics by a bacterial isolate Paenibacillus alvei

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• 作者：Balaiah An ; araj ; Adaikkalam Vellaichamy ; Maureen Kachman ; Athinarayanan Selvamanik ; an ; Shyamanta Pegu ; Vadivel Murugan
• 关键词：Paenibacillus alvei ; Peptide antibiotics ; Co-production ; Antagonistic activity ; Protease ; Mass-spectrometry analysis
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：6 February 2009
• 年：2009
• 卷：379
• 期：2
• 页码：179-185
• 全文大小：749 K

文摘

Two new peptide antibiotics were secreted by a Gram-positive bacterial strain isolated from fermented tomato fruit. Based on its 99 % 16S rDNA sequence similarity with Paenibacillus alvei, the isolate was designated as P. alvei NP75. Among these two peptides, one is active against Gram-positive pathogens while the other against Gram-negative pathogens; thus these peptides were named as paenibacillin P and paenibacillin N, respectively. After the purification of those peptide antibiotics from the cell free culture supernatant by RP–HPLC, they were analyzed for their temperature sensitivity and susceptibility to proteases. Higher-temperature tolerant paenibacillin N was easily degraded by proteinase K, while the temperature sensitive paenibacillin P was not affected by any of the proteases used in this study other than a specific protease that was secreted by the same NP75 strain. Mass-spectrometry analysis of the above peptide antibiotics further confirmed their distinction among the known peptide antibiotics. We are reporting first of its kind the co-production of two different new peptide antibiotics from a single bacterial isolate of P. alvei strain.