Curcumin loaded chitosan nanoparticles impregnated into collagen-alginate scaffolds for diabetic wound healing

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• 作者：Veera Venkata Satyanarayana Reddy Karri^a ; ^{ksnreddy87@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Gowthamarajan Kuppusamy^a ; ^{gowthamsang@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Siddhartha Venkata Talluri^a ; Sai Sandeep Mannemala^b ; Radhakrishna Kollipara^a ; Ashish Devidas Wadhwani^c ; Shashank Mulukutla^d ; Kalidhindi Rama Satyanarayana Raju^d ; Rajkumar Malayandi^e
• 关键词：Curcumin ; Chitosan ; Nanoparticles ; Collagen ; Scaffold ; Diabetic wound
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：93
• 期：part_PB
• 页码：1519-1529
• 全文大小：4179 K

文摘

Diabetic wounds are a common complication in patients with diabetes that often lead to amputation. Although the pathophysiology of diabetic wound is multifactorial, chronic inflammation and lack of tissue regeneration leads to impair wound healing in diabetes. Application of curcumin (CUR) which is a well-known anti-inflammatory and antioxidant agent could be better strategy in diabetic wound healing. However, low bioavailability and poor stability of CUR hinders its application. Hence, in present study a novel nanohybrid scaffold has been prepared by incorporating CUR in chitosan nanoparticles (CSNPs) to improve stability and solubility followed by impregnation of prepared CUR-CSNPs into collagen scaffold (nanohybrid scaffold) for better tissue regeneration application. The prepared CUR-CSNPs were evaluated for particle size, zeta potential, SEM, differential scanning calorimetry and X-ray powder diffraction studies and the novel nanohybrid is evaluated for morphology, biodegradability, biocompatibility, m>in vitrom> drug release and m>in vivom> wound healing studies. The results of NPs evaluation suggest the better stability and solubility of CUR. The nanohybrid scaffold showed good m>in vitrom> characteristics in terms of better water uptake, biocompatibility and sustained drug availability. The results of m>in vivom> wound closure analysis revealed that nanohybrid scaffold treated wounds contracted significantly (p < 0.001) faster than the wounds from the control and placebo scaffold groups. Further, the obtained results suggest that complete epithelialization with thick granulation tissue formation occur in nanohybrid scaffold group, whereas lack of compact collagen deposition in placebo scaffold group and presence of inflammatory cells in control group was observed. Hence, the present study suggests that the synergistic combination of CUR (anti-inflammatory and anti-oxidant), chitosan (sustain drug carrier, wound healing) and collagen (established wound healer as scaffold) is a promising strategy to address various pathological manifestations of diabetic wounds and have better wound healing capability.