文摘
Summary
In our attempt to characterize a general immune-suppression found in patients with squamous cell carcinoma of the head and neck (SCCHN) we now focused on a subset of CD3 lymphocytes described as γ/δ-T-cells, a cell type with potential relevance in non-MHC restricted anti-tumor immune responses. Peripheral blood of 33 SCCHN patients and 33 age-matched controls (CON) was evaluated for the frequency of γ/δ-T-cells among CD3+ T-cells and their onset of apoptosis (Annexin V binding) by multicolor flow cytometry. Results were correlated with clinical parameters. Patients with SCCHN had a significantly higher proportion of γ/δ-T-cells compared to healthy controls (4.4 ± 0.4 % for SCCHN vs. 3.0 ± 0.3 % for CON, p = 0.01). However, this increase was not paralleled with a difference in the onset of apoptosis if compared to CON. There was also no correlation between the proportion of γ/δ-T-cells and tumor stage. However, a significantly higher proportion of γ/δ-T-cells was found in patients with recurrent or metachronous second primary SCCHN (6.0 ± 1.0 % ) if compared to the other SCCHN (3.8 ± 0.4 % , p = 0.02). In a follow up 3–6 months post-treatment patients showed a decrease of γ/δ-T-cells among CD3+cells (2.7 ± 0.4 % , n = 4) if they were operated only and an increase if primary radio-chemotherapy (6.7 ± 1.7 % , n = 8) or a combination of operation plus radio-chemotherapy (6.8 ± 2.3 % , n = 3) was applied. Furthermore, patients receiving palliative treatment including radio-chemotherapy had highest values of γ/δ-T-cells (9.1 ± 2.7 % , n = 4) overall implicating that the treatment modality significantly influences the proportion of γ/δ-T-cells. Since patients with SCCHN, particularly those with recurrent or second primary disease after treatment, had a higher proportion of γ/δ-T-cells without signs of a reduced onset of apoptosis this could be due to an increased de novo generation. The current study implies that increased frequencies of γ/δ-T-cells in patients with SCCHN may not only be the result of tumor–host interactions but the consequence of applied treatment modalities.
