Denervation decreases the ipsilateral expression of AChE in chick lumbaric motor neurons
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- 作者：Wan ; David C.C. ; Ng ; Yu P. ; Choi ; Roy C.Y. ; Cheung ; Pine W.T. ; Dong ; Tina T.X. ; Tsim ; Karl W.K.
- 关键词：Motor neuron ; Muscle development ; Neuromuscular junctions ; Postsynaptic specializations
- 刊名：Neuroscience Letters
- 出版年：1997
- 出版时间：August 29, 1997
- 年：1997
- 卷：232
- 期：2
- 页码：83-86
- 全文大小：506 K
文摘
In vertebrate neuromuscular junctions, acetylcholinesterase (AChE; EC 3.l.1.7) is highly concentrated at the synaptic basal lamina and the postsynaptic muscle fiber. The postsynaptic muscle cell is the primary source of AChE. However, several lines of evidence indicate that the presynaptic motor neuron is able to synthesize and secrete AChE at the neuromuscular junctions. By using anti-AChE monoclonal antibody in immunohistochemical staining, we found that the AChE-positive cells were labeled only at the motor neurons of the chick spinal cords. When the protein extract of chick spinal cords was analyzed by a Western blot analysis, a protein band of ˜105 kDa was recognized. In denervated chicks, the expression of motor neuron AChE, as recognized on a Western blot, decreased by ˜50 % 4 days after denervation. The AChE expression in denervated chick spinal cords, however, was restored to control level 10 days after denervation. The decreased AChE expression was restricted to the ipsilateral side of the denervated chick spinal cord while the contralateral side was relatively unchanged. In comparison with the contralateral side, the level of AChE protein and enzymatic activity expressed in the ipsilateral spinal cord was ˜50 % lower. This is the first demonstration to show that the ipsilateral and contralateral sides of chick spinal cords respond differently after nerve denervation.