Factorial analysis of HS-SPME parameters is essential to quantify N-nitrosamines.
HS-SPEME-GC-MS is an excellent option to quantify N-nitrosamines in red wine.
The standard addition technique was found to be an adequate method to quantify N-nitrosamines.
HS-SPEME-GC-MS achieved good validation results when used to quantify N-nitrosamines in red wine.