Up-regulation of the novel proinflammatory adipokines lipocalin-2, chitinase-3 like-1 and osteopontin as well as angiogenic-related factors in visceral adipose tissue of patients with colon cancer

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• 作者：Victoria Catal&#xe1 ; n^a ; ^b ; Javier Gó ; mez-Ambrosi^a ; ^b ; Amaia Rodr&#xed ; guez^a ; ^b ; Beatriz Ram&#xed ; rez^a ; ^b ; Camilo Silva^b ; ^c ; Fernando Rotellar^b ; ^d ; Jos&#xe9 ; L. Hern&#xe1 ; ndez-Lizoain^d ; Jorge Baixauli^d ; Victor Valent&#xed ; ^d ; Fernando Pardo^d ; Javier Salvador^b ; ^c ; Gema Frü ; hbeck^a ; ^b ; ^c ; ^{gfruhbeck@unav.es"" rel=""nofollow}
• 关键词：Visceral adipose tissue ; Colon cancer ; Inflammation ; Angiogenesis ; Gene expression
• 刊名：Journal of Nutritional Biochemistry
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：22
• 期：7
• 页码：634-641
• 全文大小：534 K

文摘

Background

Obesity is widely recognised as an important risk factor for colorectal cancer (CC).

Aim

The study aimed to evaluate the effect of CC on circulating concentrations and gene expression levels of inflammatory and angiogenesis-related factors in human visceral adipose tissue (VAT).

Methods

VAT biopsies were obtained from 18 healthy individuals and 11 patients with CC. Real-time polymerase chain reactions were performed to quantify gene expression levels and zymographic analyses were used to determine the activity of matrix metalloproteinases (MMPs).

Results

Patients with CC exhibited increased mRNA expression levels of lipocalin-2 (P=.014), osteopontin (P=.027), tumor necrosis factor-α (TNF-α) (P=.016) and chitinase-3 like-1 (P=.006) compared to control subjects in VAT. Gene expression levels of hypoxia-inducible factor-1 α, vascular endothelial growth factor and MMP-2 were significantly higher (P<.05) in VAT of patients with CC. The expression of insulin-like growth factor I, insulin growth factor binding protein 3 and MMP-9 followed the same trend, although no significant differences were reached. The enzymatic activity of MMP-9 was increased (P<.001) in patients with CC. Furthermore, individuals with CC showed increased (P<.05) circulating concentrations of the inflammatory markers interleukin-6, tumour necrosis factor α and hepatocyte growth factor, whereas levels of the anti-inflammatory adipokine adiponectin were decreased (P<.01).

Conclusion

These findings represent the first observation that mRNA levels of the novel inflammatory factors lipocalin-2, chitinase-3 like-1 and osteopontin are increased in human VAT of subjects with CC. This observation together with the up-regulation of angiogenic factors suggests that adipokines secreted by VAT may be involved in the development of colon cancer.