Antibacterial activity of selected Cameroonian dietary spices ethno-medically used against strains of Mycobacterium tuberculosis

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• 作者：Emmanuel Mouafo Tekwu ; T¨¹lin Askun ; Victor Kuete ; Augustin Ephraim Nkengfack ; Barth¨¦l¨¦my Nyasse ; Fran?ois-Xavier Etoa ; V¨¦ronique Penlap Beng
• 关键词：TB ; tuberculosis ; ATCC ; American Type Culture Collection ; MABA ; Microplate Alamar Blue Assay ; MIC ; minimum inhibitory concentration ; MBC ; minimum mycobactericidal concentration ; WHO ; World Health Organization ; MDR ; multi-drug resistant TB ; XDR ; extensivel
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 出版时间：13 July, 2012
• 年：2012
• 卷：142
• 期：2
• 页码：374-382
• 全文大小：435 K

文摘

Ethnopharmacological relevance

Tuberculosis (TB) is considered as a re-emerging disease and one of the most important public health problems worldwide. The use or (in most cases) misuse of existint anti-tuberculosis drugs over the years has led to an increasing prevalence of resistant strains, establishing an urgent need to search for new effective agents. Spices are largely used ethno-medically across Africa.

Aim of the study

The present study aimed to evaluate the in vitro antimycobacterial activities of a total of 20 methanol crude extracts prepared from 20 Cameroonian dietary spices for their ability to inhibit the growth of or kill Mycobacterium tuberculosis strains H₃₇Rv (ATCC 27294) and H₃₇Ra (ATCC 25177).

Materials and methods

The antituberculosis screening was performed using the Microplate Alamar Blue Assay (MABA) method to determine the minimum inhibitory concentration (MIC) and the minimum mycobactericidal concentration (MBC).

Results

Fifteen (15) plant extracts out of 20 showed varied levels of antimycobacterial activity against the strains M. tuberculosis H₃₇Rv and H₃₇Ra, with MICs in the range of 2.048-0.016 mg/ml. The extract of Echinops giganteus exhibited the most significant activity with a MIC value of 32 ¦Ìg/ml and 16 ¦Ìg/ml, respectively against H₃₇Ra and H₃₇Rv. To the best of our knowledge, the antimycobacterial activity of the tested spices has not been reported before and therefore our results can be evaluated as the first report about the antimycobacterial properties.

Conclusions

The results of this study suggest that Echinops giganteus and Piper guineense could be important sources of bactericidal compounds against M. tuberculosis and could probably be promising candidates that can be further investigated.