One-pot preparation of SBP-PANI-PAA-ethylene glycol diglycidyl ether sensor for electrochemical detection of H2O2
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- 作者：Dimas Ignacio TorresaAuthor Vitae ; Marí ; a Victoria MirandabAuthor Vitae ; Viviana Campo Dall&rsquo ; Ortoa ; vcdall@ffyb.uba.ar" class="auth_mail" title="E-mail the corresponding author ; vivianacdall@gmail.com" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Polyaniline ; Soybean seed coat peroxidase ; Epoxy ; Poly(acrylic acid) ; Hydrogen peroxide
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：239
- 期：Complete
- 页码：1016-1025
- 全文大小：1954 K
文摘
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A one-pot strategy of a soybean peroxidase-PANI biosensor construction for operation at neutral pH is proposed.
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Acrylic acid contributes to obtain PANI in the conducting emeraldine salt form and to entrap the enzyme in a hydrogel matrix.
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Soybean seed coat peroxidase is used as catalyst in the formation of the matrix and as sensing element.
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The biosensor exhibits H2O2 detection sensitivity of 0.0875 nA μM−1 with a LoD of 2.2 μM at pH 7.0 in the flow system.