Differential identification of Chlamydophila abortus live vaccine strain 1B and C. abortus field isolates by PCR-RFLP

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• 作者：Karine Laroucau ; Fabien Vorimore ; Konrad Sachse ; Evangelia Vretou ; Victoria I. Siarkou ; Hermann Willems ; Simone Magnino ; Annie Rodolakis ; Patrik M. Bavoil
• 关键词：Chlamydophila abortus ; Live vaccine ; Identification
• 刊名：Vaccine
• 出版年：2010
• 出版时间：9 August 2010
• 年：2010
• 卷：28
• 期：35
• 页码：5653-5656
• 全文大小：195 K

文摘

Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydophila abortus and its nitrosoguanidine-induced, temperature-sensitive and virulence-attenuated live vaccine derivative identified point mutations unique to the mutant (Burall et al. [1]). Here, we evaluate the capacity of some of these mutations to either create or eliminate restriction sites using the wild-type strain C. abortus S26/3 as a reference. Three of eight genomic sites with confirmed point mutations (CAB153, CAB636 and CAB648) were retained for analysis as each resulted in the loss of a restriction site in the genome sequence of the vaccine strain. PCR-restriction fragment length polymorphism analysis using restriction enzymes chosen to specifically target the three genomic sites was then applied to a large number of C. abortus field isolates and reference strains. Our results indicate that the three mutations are uniquely present in the vaccine strain, and as such provide easy-to-use markers for the differential identification of the vaccine strain and wild-type isolates.